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Of the temperature dependent markers the most important is the ratio of 18O to 16O. A major disadvantage of these types of dating is that they are extremely time consuming. Thus, water evaporating from the ocean it starts off H2(18O) poor. The water molecules composed of H2(18O) evaporate less rapidly and condense more readily then water molecules composed of H2(16O). Both of these isotopes are produced by cosmic rays and solar irradiation impinging on the upper atmosphere, and both are quickly washed from the atmosphere by precipitation.
These inclusions are typically ash from volcanic eruptions and acidic layers.The major advantage of these methods is that they can be completed relatively quickly.